FastQCFastQC Report
Sat 12 Dec 2020
H7LNCBGXH_n01_AKM144.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7LNCBGXH_n01_AKM144.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17340811
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC15551698.968259904337808TruSeq Adapter, Index 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG312260.1800723161102442No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATGC279130.16096709663694508TruSeq Adapter, Index 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1746350.067.6398544
GTATGCC1748000.067.5914445
TATGCCG1747250.067.5639746
TCGTATG1755350.067.3405743
GCCGTCT1729850.067.3212949
ATGCCGT1744250.067.3154547
CTCGTAT1713400.067.30681642
TGCCGTC1745750.067.06801648
CCGTCTT1741950.066.8295550
CACGATC1685100.066.6700336
CGATCTC1696300.066.6672538
ACGATCT1690250.066.6588937
GATCTCG1704600.066.6438939
TCTCGTA1716300.066.6429141
CTGCTTG1677600.066.440357
TCACGAT1736600.066.1477235
ATCTCGT1722800.066.0027740
CATCACG1773350.065.7783333
CTTCTGC1767800.065.7483554
ATCACGA1761200.065.7418134
Produced by FastQC (version 0.11.5)