FastQCFastQC Report
Fri 8 Jul 2016
H7LCCBGXY_n01_ve59.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7LCCBGXY_n01_ve59.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71285399
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA179400.020.8332273
GCAGCGT179450.020.7292421
CAGCGTC183900.020.3839452
GCGTCAG189350.019.664334
CTAAGGC1641500.019.48415670
TAAGGCG1147400.018.73654670
ACTAAGG2194300.018.30602570
CGTCAGA234850.015.8544935
GACTAAG2868950.015.62485970
TATGCCG136550.015.4033843
AAGGCGA903850.014.94952270
GCCGTCT139000.014.82929146
AGGCGAA679950.014.29297770
AGACTAA3680900.013.28683970
ATGCCGT164050.012.88516644
CGTATGC165750.012.88184641
TCGTATG168450.012.40612740
TGCCGTC173050.012.25529145
GTATAAG336400.012.03675615
GGCGAAT532450.011.92291170