FastQCFastQC Report
Fri 8 Jul 2016
H7LCCBGXY_n01_ve58.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7LCCBGXY_n01_ve58.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7758602
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGAC11150.011.6128713
TATACTG36300.011.1830785
TACACCG16300.011.1641525
GTGTAAG18200.011.163291
GCTGTCC17800.010.8136488
CTATACT18800.010.7964984
TACACTG23500.010.7219715
GTGTTAG15950.010.5418341
GTAGGGC8700.010.4584393
ACTGTCC15750.010.443528
TACACAG20850.010.4062825
GTATAGG12250.010.2944231
CCACGAG64450.010.26259770
AGGGGTG16400.010.2425335
GTGCTAG9250.010.2248661
GTTCTGG38450.010.2036911
GTCCAGG13400.010.1951921
ACCGTGC14550.010.1021998
TCTACAC28900.010.0506243
GGGGTGT14000.09.9985986
Produced by FastQC (version 0.11.2)