FastQCFastQC Report
Fri 8 Jul 2016
H7LCCBGXY_n01_ve57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7LCCBGXY_n01_ve57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11537412
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGCGT30100.012.9167021
GTATAAG34300.011.42741715
GTCTTAG14500.011.3533751
GTATATA41950.011.1883991
AGCGTCA34050.010.9970873
ACCACGT40750.010.7349728
TACACCG24800.010.7241655
ACCCGTT51700.010.62741570
AGACCCG65100.010.42891870
CCCCTGG12800.010.3984531
TAGACTG20900.010.38118655
TACACAG28350.010.3687835
GACCCGT53000.010.23472669
TACACTG35200.010.1404935
TATACTG54050.010.1002115
GCGTCAG37550.010.06512454
TATACAG34250.010.0130655
GTGTTAG23450.010.0075341
GTGTAGG25300.09.96797851
CTACACT34950.09.9127324