FastQCFastQC Report
Fri 8 Jul 2016
H7LCCBGXY_n01_ve56.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7LCCBGXY_n01_ve56.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16469612
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC30650.013.01633553
CTATACT35600.012.8774654
CTGGATC141600.012.35737970
TACACTG54350.011.6542815
GCTATAC19300.011.2421333
ACTGGAT173200.011.15349170
ACTGTCC35250.011.119438
TATACAG46700.011.0155695
AGACTGG199300.010.95713270
CTGTCCC31800.010.8951119
AGGGGTG33600.010.51934055
GACTGGA189700.010.51543270
GGACTGC19800.010.4277826
GTATATA58100.010.3097921
CTACACT54950.010.0623274
GTATAGA36250.010.0497481
CAGGGGT31350.010.0464834
TACACCG28250.010.0339755
GTATATG43300.09.9505511
TAGACAG36700.09.9168495