FastQCFastQC Report
Fri 8 Jul 2016
H7LCCBGXY_n01_ve55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7LCCBGXY_n01_ve55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9573561
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG15850.014.807081
CTATACT20250.014.3439424
GTATAGA22350.013.7920591
AGGGGTG20150.013.1992565
TACACCG15250.012.8507685
CACCACT86900.012.76613770
TACACTG30650.012.6736995
ACCACTG65100.012.57925370
TAGACAG19150.012.4265635
GTTCTGG55500.012.3073781
CTGTCCC18000.012.2483889
ACACCAC108050.012.24297670
TCTATAC20300.012.2401533
TATACAG25550.012.1901785
CAGGGGT22850.012.0991914
TACACAG26700.012.0583385
CTGTGCG15650.011.8514769
GTCCTAC9250.011.7393451
GACACCA127500.011.66537670
CTACACT30400.011.6268824