FastQCFastQC Report
Fri 8 Jul 2016
H7LCCBGXY_n01_ve54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7LCCBGXY_n01_ve54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12403515
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG42950.014.9103335
TATACAG36250.013.8047145
GTATAGG24700.013.6147561
CTGTCCC23600.013.49379359
GTATTAG28300.013.4919791
GTCCTAC11700.013.4729351
TATACTG52050.013.1103195
CTATACT30600.013.0372084
CTACACT40050.013.01921754
CTTACAC27200.012.9944273
GTCTTAG20550.012.7845371
TCTATAC28750.012.6590223
TACACAG34350.012.530775
GTATAAG26300.012.5200971
CCCCTGG11200.012.5105821
GCTATAC16200.012.3130173
CCTACAC28750.012.2938583
TACACCG20350.012.20937255
GTGTAGA50000.012.1202531
AGGGGTG27450.012.1110185
Produced by FastQC (version 0.11.2)