FastQCFastQC Report
Fri 8 Jul 2016
H7LCCBGXY_n01_ve53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7LCCBGXY_n01_ve53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12120004
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTC45700.014.31995170
ACGGACT60800.013.64146170
GGACTCC35550.013.58487470
GACGGAC76900.013.37941570
TACACTG39350.012.8949515
GTATAGA31000.012.5440081
GTCCTAC12200.012.3476321
ACTGTCC25900.012.1605588
GTATTAG26450.011.9204281
TATACTG48350.011.7974345
GTGTAGG25750.011.4281811
CCTATAC21450.011.4203113
CTGTCCC22700.011.4081879
CTATACT28650.011.3594764
GTATAGG21400.011.2956281
GTGTAGA41900.011.2874231
TAGACAG26100.011.262515
TACTCCG17950.011.1123735
GCAGCGT22100.011.0963671
TACACAG32350.010.9255545
Produced by FastQC (version 0.11.2)