FastQCFastQC Report
Fri 8 Jul 2016
H7LCCBGXY_n01_ve52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7LCCBGXY_n01_ve52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16035820
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTCCT148950.014.44962470
TACACTG49250.014.0687845
CTCCTGA114550.013.25917870
TAGACAG35750.013.2146395
CTATACT34700.013.1103064
ACTGTCC33300.012.9260728
TACACCG27400.012.8993675
GTATTAG32200.012.7266781
TATACTG60800.012.489755
GTCCTAA24400.012.4886211
ACTCCTG146900.012.41188970
TCCTGAG79250.012.32045970
CTTACAC35050.012.2806533
GTATAGG27700.012.2652661
GTGTAGA56950.012.11595251
GTATAGA37500.012.0487921
AGGGGTG30500.011.932495
GTGTAGG31600.011.85992051
AGACTCC181850.011.85474269
ACAGTGC43850.011.8113028
Produced by FastQC (version 0.11.2)