FastQCFastQC Report
Fri 8 Jul 2016
H7LCCBGXY_n01_ve51.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7LCCBGXY_n01_ve51.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8589068
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGG101900.019.71395770
ACGTGTG129200.015.84635870
GTGTGGT78700.015.03066170
GTGTAGG18600.014.6896811
CTAAGAC9400.013.7747813
GACGTGT159850.013.57422970
CTATACT16600.013.4920424
GTACTAG10450.012.4026981
GTATTAG14750.012.3492921
GTTCTAG21550.012.1911511
TACACTG23000.012.1721695
TAGGACC6650.012.1035284
CCTATAC13800.011.9187193
AGACGTG193150.011.85002270
GTATATG19600.011.7955611
GTGCTAC14550.011.5560381
TACACCG26400.011.5324375
GTGCTAG7900.011.528611
GTATAGA15850.011.4922431
GCTATAC9800.011.4270663
Produced by FastQC (version 0.11.2)