FastQCFastQC Report
Fri 8 Jul 2016
H7LCCBGXY_n01_ve50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7LCCBGXY_n01_ve50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5290514
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGGGG54650.021.45283570
GAGGGGT39350.019.9220270
ACGAGGG71250.016.4055869
GCAGCGT9200.013.3263971
GTCTTAG8250.013.1625561
GTTCTAG18650.012.3964581
GTGCTAG5950.012.3633121
GACGAGG98350.012.16975468
ACCGCGT5257.2759576E-1111.9984968
TACACTG16800.011.87345
CTATACT11250.011.8208534
GTGTAGA19600.011.79560851
CCTACAC12900.011.6654243
AGACGAG114650.011.53845870
AGGGGTT29450.011.52700570
TATACCG8750.011.1984915
GTGTTAG10400.011.1150931
GTAAGAC9900.010.9584293
GTAGGAC5854.783942E-1010.7680853
TAAAGGC13000.010.76798254
Produced by FastQC (version 0.11.2)