FastQCFastQC Report
Fri 8 Jul 2016
H7LCCBGXY_n01_ve49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7LCCBGXY_n01_ve49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8096152
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG14850.014.1393855
TATACTG51550.013.3055595
AGACTGC53400.013.04176470
CGAGACT74150.013.02634170
GTATTAG50800.012.9624251
CCCTTAG18450.012.7195161
GAGACTG77100.012.70949270
CACGAGA108100.012.59355770
CCACGAG127950.012.52707670
ACGAGAC90000.012.48208370
GACTGCT43400.012.41811170
CTATACT40050.012.23325354
CCTATAC32350.011.7916363
CCTAGTA23650.011.6918212
TACACTG47900.011.4701715
TAGGCTG28700.011.461775
GTATAGG31050.011.3933621
CTAAGAC33600.011.3529593
CTAATAC49700.011.2664073
TGTCCCG14600.011.26599510
Produced by FastQC (version 0.11.2)