FastQCFastQC Report
Fri 8 Jul 2016
H7LCCBGXY_n01_ve48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7LCCBGXY_n01_ve48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2425217
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGAG25700.016.47666270
ATAGGAC2552.18497E-816.4672953
TGCTAGG1901.1348764E-414.73662952
GTAGAGG21550.014.61545270
CTATAGG3604.620233E-1014.59275151
TAGACCG3151.861008E-814.4415565
ACGTAGA37850.014.42366870
TGTCCCA7250.013.99777710
GACGTAG39150.013.85533269
CTTACAC4901.8189894E-1213.5687153
CTAGGGG1550.007387287413.5456783
GTCCTAT2607.850396E-613.4702321
CTATACT5250.013.3306674
TACACCG5001.8189894E-1213.2973415
CTGGTCA5550.013.241149
TAGAGGA18250.013.23132170
GTGTAGC5300.013.2160771
GTCCCAT6150.013.08762311
CTAAGCG2153.243633E-413.0206513
TAAGACG3507.4127456E-812.99744