FastQCFastQC Report
Fri 8 Jul 2016
H7LCCBGXY_n01_ve47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7LCCBGXY_n01_ve47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4415836
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACAC6000.018.081253
GTATAAG7550.017.61406515
AGAGAGG76850.017.07753270
CCAGAGA153500.015.36700470
ACCAGAG175550.015.33074370
TACACCG10650.014.7868615
AGAGGAT38850.014.23323970
GTCCTAC6900.014.2152661
CAGAGAG144900.014.12944770
TACACAG6000.013.9982285
CCTATAC5400.013.6095433
GTAGGAC6200.013.5468273
GTATAGG4002.235538E-913.136431
CTTAGAC4002.2682798E-913.1234883
GAGAGGA60250.013.06962670
TATACAG7900.012.8464765
GTGTATA10900.012.84299413
GACCAGA216450.012.48252269
CTATACT6200.012.4177854
CCTACAC10750.012.3706673
Produced by FastQC (version 0.11.2)