FastQCFastQC Report
Fri 8 Jul 2016
H7LCCBGXY_n01_ve46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7LCCBGXY_n01_ve46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14544046
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG34300.024.58972215
GTGTATA38750.022.48846613
TCTCGTA59700.020.04797470
CTCTCTA689950.019.7462970
GATGTGT45950.019.04020910
CCTCTCT953150.018.59307370
ATGTGTA49050.018.26537711
ATAAGAG48100.018.11756717
TAAGAGA48900.018.10707518
TCTCTAC498400.017.79994670
TACACCG29050.017.7082865
GTCAGAT50100.017.3926966
TATAAGA49100.017.2495616
CTCTACA368700.016.75291370
GAGACAG51100.016.6424621
TGTGTAT54100.016.62524412
CGTCAGA51000.016.6054445
TCTACAT275400.016.58308470
AGATGTG53350.016.3991499
TGTATAA54250.016.32134414