FastQCFastQC Report
Fri 8 Jul 2016
H7LCCBGXY_n01_ve45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7LCCBGXY_n01_ve45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9591532
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGCA169150.014.68937170
AGGCATG115150.012.8860270
ACTAGGC206700.012.76579270
TCTATAC21900.012.30435853
TAGGCAT150900.012.19869570
GCATGAT75200.011.820415570
CTATACT21650.011.6379884
GGCATGA88550.011.14493270
GATCTCG37500.011.01203570
GACTAGG242250.010.9357670
TATACAG25300.010.7887935
AGACTAG287600.010.39168370
GCCCTAT10450.010.3905971
GTACTAG13600.010.0443281
TATCCCG13300.09.99840455
ACTGTGC23200.09.8048288
GTATTAG19800.09.72954751
GTATAGA22950.09.6150821
TAAGGTG15000.09.565145
TAGGGCA11350.09.2497224
Produced by FastQC (version 0.11.2)