FastQCFastQC Report
Fri 8 Jul 2016
H7LCCBGXY_n01_ve44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7LCCBGXY_n01_ve44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23489713
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGCAG588550.013.71213270
AGGCAGA469500.012.95517370
ATCTCGT110700.012.61404470
ACAGGCA697050.012.16015870
AATCTCG138350.011.48433270
GGCAGAA480250.010.93082470
GTATTAG52250.010.8598791
GACAGGC803550.010.82722870
GTATAGA58500.010.6576231
AGACAGG964550.010.62731870
GTATATA98400.010.5720111
CTATACT58000.010.4380284
TAGACAG51050.010.1453125
CCTATAC45300.09.9653573
GTATACG37800.09.7295721
GTGTAAG47850.09.5892711
GAGACAG1138300.09.56164670
GCAGAAA416300.09.54154170
CGAGACA1303450.09.50200570
GTATATG78600.09.4919021