FastQCFastQC Report
Fri 8 Jul 2016
H7LCCBGXY_n01_ve43.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7LCCBGXY_n01_ve43.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5725664
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACT43950.018.39474370
CGTACTA29150.016.5684270
GCAGCGT9250.015.9049181
ACCGTAC55950.014.32438669
GTACTAG23350.014.089046570
AGCGTCA11400.013.5070933
CAGCGTC11250.013.0673262
GACCGTA61650.012.82968568
GCCTTAG5305.456968E-1212.5574541
GCGTCAG11950.012.299624
AGACTGC10100.012.12716
GCCCTGG5501.2732926E-1112.1008191
AGACCGT75800.011.86600670
GTCTTAG7800.011.67622951
AGGGGTG10050.011.4910095
CTACGCA5602.237357E-1011.2485044
GTGCTAG6108.367351E-1110.9105751
TAAGGGT8700.010.8606244
CTGTCCC9100.010.7678939
CAGGGGT10100.010.7411454
Produced by FastQC (version 0.11.2)