FastQCFastQC Report
Fri 8 Jul 2016
H7LCCBGXY_n01_ve42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7LCCBGXY_n01_ve42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21086462
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGAGG131950.016.20512270
CTATACT55950.016.0120894
TACACCG39550.015.5730355
TACACTG79450.015.416345
ACCACGT71000.014.6882198
ACTGTGC59200.014.5420178
GTATAGG47550.014.5119111
GTATTAG55050.014.5072951
ACCGTGC23000.014.3024768
ACTGTCC46050.014.2869468
TATACCG38550.014.2522155
TATACAG76750.014.2260175
TCTATAC56350.014.0977023
GTGTAGC40200.013.9412861
TAGACTG52150.013.8235655
TACACAG63850.013.7020715
TCTACAC85500.013.6299583
GTATAAG50500.013.4561011
CTTACAC52300.013.4496433
GTGTATA69650.013.2767481
Produced by FastQC (version 0.11.2)