FastQCFastQC Report
Fri 8 Jul 2016
H7LCCBGXY_n01_ve41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7LCCBGXY_n01_ve41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16680685
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG208840.12519869537731815No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC202110.12116408888483896No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGAGG88200.014.87953370
CCGAGGC66200.014.69648570
TACACTG64250.013.7800855
CTATACT45100.013.4237974
TACACAG48450.013.0011945
TATACCG29650.012.7468715
ACTGTGC49450.012.6676998
AGACCGA142800.012.64585370
TACACCG30550.012.3713495
TATACTG84600.012.2440715
ACTGTCC37700.011.9745568
GACCGAG112000.011.84262170
GTACTAG31400.011.8239421
GTATATG57500.011.573691
TCTACAC61550.011.541983
TATACAG54450.011.4400165
GTGTAGA65950.011.365411
GTATTAG43900.011.3294791
ACCGTGC22900.011.3085588
GAGACCG175950.011.23791770
Produced by FastQC (version 0.11.2)