FastQCFastQC Report
Fri 8 Jul 2016
H7LCCBGXY_n01_ve40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7LCCBGXY_n01_ve40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15582316
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG61000.018.5297225
CTATACT44600.016.7909474
ACGCTAC61850.016.46564770
CGCTACG48450.015.89111770
GCTACAC31050.015.6658883
TCTATAC39200.015.5333453
ACCACGT55100.015.4333688
GACGCTA79800.015.30551970
CTGTCCC32500.015.2901739
GTGTAGC31100.014.9799361
GTGTACG26100.014.6286641
GTGTAGG40950.014.627191
CCTATAC37400.014.5031373
TAGACAG41650.014.4513935
GTATAGG32850.014.395181
TACACCG27100.014.3334245
GCTACGC31650.014.26400770
GCTGTCC32900.014.2532838
TACACAG44200.014.2510385
ACTGTGC44950.014.247138
Produced by FastQC (version 0.11.2)