FastQCFastQC Report
Thu 30 Jun 2016
H7L3YBGXY_n01_js_tga1bzip1_9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7L3YBGXY_n01_js_tga1bzip1_9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23086562
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGG529990.22956644648952063No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC522540.22633946102498934TruSeq Adapter, Index 9 (100% over 50bp)
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACA374490.16221124652514307No Hit
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACG312220.13523884587059778Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACA140800.053.28388210
TCCCTAC144650.051.79219
CTACACG154200.047.17867312
CCTACAC163900.045.85967311
TACACGA156800.045.61657713
TATGCCG89950.042.1818946
ACACGAC165400.041.84837714
GTATGCC91800.041.52012645
GACGCTC160650.040.84089318
CGTATGC93400.040.73417744
TTCCCTA197500.038.7104268
CACGACG183000.036.84854515
ATGCCGT104100.036.7519647
ACGCTCT181650.036.7008119
TCGTATG106450.035.77535643
CTCGTAT104750.035.52049642
ACACTCT216850.034.769791
CGCTCTT194650.034.3750220
CACTCTT228900.033.0059932
CTTTCCC238150.032.3525476
Produced by FastQC (version 0.11.2)