FastQCFastQC Report
Thu 30 Jun 2016
H7L3YBGXY_n01_js_tga1bzip1_8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7L3YBGXY_n01_js_tga1bzip1_8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20288873
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACG574520.2831699917486792Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC553000.2725631926425879TruSeq Adapter, Index 8 (100% over 50bp)
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGG459270.22636545657316698No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340910.16802806148966482No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACA329210.1622613537972267No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACA213000.057.23473410
TCCCTAC216200.056.3711369
CTACACG224750.053.1060712
TACACGA225400.052.4251113
CCTACAC232950.052.40834811
ACACGAC235700.049.4512514
GACGCTC237300.047.58379718
TTCCCTA261000.047.4037328
CACGACG254650.045.22131315
GTATGCC105700.044.30628645
ACACTCT276300.044.2089841
TATGCCG104300.044.03011746
ACGCTCT259000.044.0204219
CGTATGC107400.042.8559444
CACTCTT289750.042.1232262
CGCTCTT273850.041.68445220
CTTTCCC300700.041.680196
CTCGTAT113550.039.88896642
TTTCCCT313250.039.8760877
ATGCCGT112150.039.7633847
Produced by FastQC (version 0.11.2)