FastQCFastQC Report
Thu 30 Jun 2016
H7L3YBGXY_n01_js_tga1bzip1_7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7L3YBGXY_n01_js_tga1bzip1_7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18697717
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC668810.3576960759433892TruSeq Adapter, Index 7 (100% over 50bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACG578980.30965277739522956Illumina Single End PCR Primer 1 (96% over 32bp)
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGG424070.22680309045216587No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACA294380.1574416812491065No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246770.13197867953611664No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACA203050.057.66305510
TCCCTAC207550.056.597839
CTACACG214900.053.18089312
CCTACAC220450.053.12775411
TACACGA215550.052.38739413
ACACGAC225800.048.89338714
TTCCCTA244600.048.6238868
GACGCTC224450.047.7683618
CACGACG235450.046.22081415
ACACTCT260450.045.195431
ACGCTCT239200.045.13384619
CTTTCCC275850.043.407456
CACTCTT273900.042.7355462
CGCTCTT256500.042.3081420
TATGCCG118150.041.5059546
TTTCCCT290200.041.2243967
GTATGCC120700.041.12030445
CGTATGC119450.041.1109744
ACTCTTT296650.039.727043
ATGCCGT125700.038.62413447
Produced by FastQC (version 0.11.2)