FastQCFastQC Report
Thu 30 Jun 2016
H7L3YBGXY_n01_js_tga1bzip1_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7L3YBGXY_n01_js_tga1bzip1_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16980487
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC729300.42949298215062975TruSeq Adapter, Index 6 (100% over 50bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACG517480.30474979898986404Illumina Single End PCR Primer 1 (96% over 32bp)
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGG404000.23792014916886658No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACA274350.16156780426851125No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263950.1554431271611939No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC175320.103247922159123TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACA174400.056.9215710
TCCCTAC179500.055.576569
CCTACAC189500.052.62607211
CTACACG187500.051.97437312
TACACGA189300.051.11256813
ACACGAC192200.049.08525514
TTCCCTA214000.047.4315458
GACGCTC197100.046.46061718
CACGACG203700.045.73030515
TATGCCG118650.045.10732346
CGTATGC120250.044.67955444
GTATGCC121350.044.1882845
ACGCTCT212150.043.6799819
ACACTCT233650.043.022211
CTTTCCC241550.042.383876
ATGCCGT128800.041.68953347
CGCTCTT224050.041.5615920
CACTCTT241300.041.4743042
CTCGTAT129950.041.4274942
TCGTATG131200.041.0592343
Produced by FastQC (version 0.11.2)