FastQCFastQC Report
Thu 30 Jun 2016
H7L3YBGXY_n01_js_tga1bzip1_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7L3YBGXY_n01_js_tga1bzip1_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17943734
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC504830.28134055041163675TruSeq Adapter, Index 5 (100% over 50bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACG465950.2596728194923086Illumina Single End PCR Primer 1 (96% over 32bp)
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGG401580.22379957259732003No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT306280.17068911075030427No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACA279340.1556755132460167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACA178950.055.1210910
TCCCTAC181400.054.6847959
CTACACG188800.051.152312
CCTACAC193850.051.0109311
TACACGA189650.050.51881813
ACACGAC194100.047.68427314
TTCCCTA218050.045.9728248
GACGCTC204050.044.14037718
CACGACG210000.043.6076915
TATGCCG111150.043.33247846
ACACTCT232950.043.1384121
CGTATGC112850.042.92640744
GTATGCC114500.042.6439645
CACTCTT240600.041.6829072
ACGCTCT218850.041.62225719
CTTTCCC251950.040.620516
ATGCCGT118350.040.2834547
CGCTCTT230900.039.58563220
TCGTATG119250.039.48019843
TTTCCCT260550.039.0110557
Produced by FastQC (version 0.11.2)