FastQCFastQC Report
Thu 30 Jun 2016
H7L3YBGXY_n01_js_tga1bzip1_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7L3YBGXY_n01_js_tga1bzip1_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16614558
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC520740.31342392617366044TruSeq Adapter, Index 4 (100% over 50bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACG379490.22840812256335677Illumina Single End PCR Primer 1 (96% over 32bp)
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGG352780.21233185980632166No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT274250.16506608240797016No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACA251770.1515357796457781No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACA156450.055.2899910
TCCCTAC159250.054.3613139
CTACACG166400.050.658912
CCTACAC172000.050.413311
TACACGA168200.049.6387313
ACACGAC172600.046.85278314
TTCCCTA196600.044.6730428
GACGCTC179700.043.42379818
TATGCCG90900.043.24265746
CACGACG187300.042.4654115
GTATGCC93650.042.0830845
CGTATGC95700.041.2186844
ACGCTCT193900.040.91488619
ACACTCT210750.040.7902151
CACTCTT213850.040.1228142
CTTTCCC223600.039.4817926
ATGCCGT99950.039.2580347
CGCTCTT204900.038.61590220
TCGTATG102300.038.45908443
TTTCCCT232200.038.01957
Produced by FastQC (version 0.11.2)