FastQCFastQC Report
Thu 30 Jun 2016
H7L3YBGXY_n01_js_tga1bzip1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7L3YBGXY_n01_js_tga1bzip1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16898166
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC585210.3463156889333434TruSeq Adapter, Index 3 (100% over 50bp)
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGG360560.21337226773603715No Hit
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACG270500.16007654321776693Illumina Single End PCR Primer 1 (96% over 32bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268160.15869177755739883No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACA259060.1533065777670784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACA118850.054.7329710
TCCCTAC123250.053.0048879
CTACACG128600.049.16853712
TACACGA127050.048.97068813
CCTACAC135450.048.49035611
TATGCCG100250.044.936146
ACACGAC136150.044.56670814
CGTATGC102800.043.92137544
GTATGCC104200.043.5331945
TTCCCTA163000.040.7867778
GACGCTC142650.040.7448918
ATGCCGT110350.040.6658447
CTCGTAT108700.040.02546342
TCGTATG112400.039.61109543
CACGACG150750.039.4614715
ACGCTCT157900.037.49984419
TGCCGTC118100.037.43455548
ACACTCT176150.036.9779741
CTTTCCC185400.036.594876
CGCTCTT167950.035.5259220
Produced by FastQC (version 0.11.2)