FastQCFastQC Report
Thu 30 Jun 2016
H7L3YBGXY_n01_js_tga1bzip1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7L3YBGXY_n01_js_tga1bzip1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19915391
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC628340.3155047269722196TruSeq Adapter, Index 2 (100% over 50bp)
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGG449930.22592074642169968No Hit
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACG399530.20061368616865216Illumina Single End PCR Primer 1 (96% over 32bp)
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACA302750.15201810499226454No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258200.12964847137573146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACA153500.055.36938510
TCCCTAC157150.054.2834859
CTACACG165500.050.1076712
CCTACAC170500.049.82841511
TACACGA169550.048.18953713
ACACGAC174700.045.68747314
TATGCCG111250.045.3371446
CGTATGC111900.045.00975844
GTATGCC113350.044.89670645
GACGCTC176050.043.58924518
TTCCCTA199900.043.303318
ATGCCGT119650.041.9503347
CTCGTAT121550.040.91950642
CACGACG191650.040.8258615
TCGTATG122050.040.55122443
ACGCTCT195300.039.56683719
ACACTCT218350.039.145281
TGCCGTC126000.039.00404448
CTTTCCC233500.037.566586
CACTCTT226900.037.476252
Produced by FastQC (version 0.11.2)