FastQCFastQC Report
Thu 30 Jun 2016
H7L3YBGXY_n01_js_tga1bzip1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7L3YBGXY_n01_js_tga1bzip1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21447545
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC519880.24239604113198038TruSeq Adapter, Index 1 (100% over 50bp)
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGG466670.2175866748385421No Hit
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACG432680.2017387071573926Illumina Single End PCR Primer 1 (96% over 32bp)
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACA323670.15091237715085806No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT307630.14343366571791782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACA180350.054.8313410
TCCCTAC183750.053.911499
CTACACG189250.051.5871112
CCTACAC196800.050.3014711
TACACGA195650.049.4707213
ACACGAC200950.046.72094314
GACGCTC205150.043.9743518
TTCCCTA229450.043.8437278
CACGACG215200.042.8961715
TATGCCG96500.041.05900646
GTATGCC96100.041.04645545
CGTATGC96650.041.03130744
ACGCTCT221400.040.98682819
ACACTCT254550.039.4802441
CTTTCCC260000.039.3114366
CACTCTT262700.038.4049152
CGCTCTT238500.038.39977320
TTTCCCT276300.036.9920467
ATGCCGT108700.036.54894647
CTCGTAT106850.036.29725642
Produced by FastQC (version 0.11.2)