FastQCFastQC Report
Thu 30 Jun 2016
H7L3YBGXY_n01_js_tga1bzip1_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7L3YBGXY_n01_js_tga1bzip1_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23429464
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC682760.29141084917691673TruSeq Adapter, Index 12 (100% over 50bp)
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGG486470.20763172388408033No Hit
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACG480710.20517328095939366Illumina Single End PCR Primer 1 (96% over 32bp)
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACA354850.1514545958029599No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACA189800.054.92146710
TCCCTAC196850.053.274029
CCTACAC209700.049.64291811
CTACACG203950.049.54994612
TACACGA201950.049.3488413
ACACGAC215250.045.24304214
TTCCCTA243050.043.8081328
GACGCTC214850.043.43721418
TATGCCG117250.043.43653546
CGTATGC120150.042.7068644
GTATGCC121200.042.39454345
CACGACG233800.040.89034315
ACACTCT263550.039.9236371
ACGCTCT238050.039.78082719
CTCGTAT124750.038.9736842
ATGCCGT130750.038.73793847
TCGTATG133600.038.17381743
CGCTCTT252300.037.70019520
CACTCTT278500.037.6828042
TGCCGTC135900.037.16763348
Produced by FastQC (version 0.11.2)