FastQCFastQC Report
Thu 30 Jun 2016
H7L3YBGXY_n01_js_tga1bzip1_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7L3YBGXY_n01_js_tga1bzip1_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20610002
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC702320.34076658507844876TruSeq Adapter, Index 11 (100% over 50bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACG520990.25278503126782814Illumina Single End PCR Primer 1 (96% over 32bp)
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGG427990.20766130930021257No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACA318850.1547064381653141No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACA185450.056.98155210
TCCCTAC190700.055.577579
CTACACG196400.052.68234312
TACACGA198100.051.8257513
CCTACAC205150.051.4928411
ACACGAC208950.048.1465814
GACGCTC208250.046.7113518
TATGCCG115700.045.9234946
TTCCCTA233550.045.873138
GTATGCC118250.044.7230445
CGTATGC119100.044.5513344
ACGCTCT224200.043.72127519
CACGACG226650.043.63001615
ACACTCT256850.041.9032061
ATGCCGT127150.041.76130747
CTCGTAT127850.041.2851542
TCGTATG130100.040.8401943
CGCTCTT244900.040.4394820
CTTTCCC269350.040.1136676
CACTCTT268750.039.9330182
Produced by FastQC (version 0.11.2)