FastQCFastQC Report
Thu 30 Jun 2016
H7L3YBGXY_n01_js_tga1bzip1_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7L3YBGXY_n01_js_tga1bzip1_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20748960
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGG485790.234127397228584No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC419910.20237640826335393TruSeq Adapter, Index 10 (100% over 50bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACG352820.170042257539655Illumina Single End PCR Primer 1 (96% over 32bp)
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACA350200.16877954364941666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACA145800.054.2893510
TCCCTAC147300.053.7597439
CTACACG160050.048.51569712
CCTACAC165850.047.85289411
TACACGA160650.047.44213
ACACGAC168800.043.2650214
TATGCCG78200.042.6119346
GTATGCC79500.042.52978545
TTCCCTA189200.042.4070858
CGTATGC78350.042.395344
GACGCTC167150.041.7654518
ACGCTCT183250.038.44251619
CACGACG187150.038.34995315
ACACTCT212450.037.7632521
ATGCCGT88600.037.13644847
CGCTCTT195900.036.42407620
CTCGTAT91450.036.05531342
CACTCTT223450.035.9877322
CTTTCCC228200.035.6349376
TCGTATG90400.035.50626843
Produced by FastQC (version 0.11.2)