FastQCFastQC Report
Thu 30 Jun 2016
H7L3YBGXY_n01_evbztga_21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7L3YBGXY_n01_evbztga_21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12554379
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC476240.3793417420327999No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG441270.3514869194246884No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT325320.25912870720248293No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA301880.24045793105337987No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG266450.21223670242869042No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG219200.17460043224758467No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC209980.16725638121965253No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG207020.16489863815645522No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC186840.14882456551614381No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC181970.14494544094932932No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG181920.14490561420839693No Hit
CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGGATG161150.12836158602508335No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT149910.11940853466348275No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA148910.11861199984483503No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG148510.11829338591737593No Hit
GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAA148230.11807035616815456No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC146900.11701096485935306No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT142760.11371331071015141No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA141280.11253443917855276No Hit
GTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCAT138210.11008907728530419No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT136010.10833670068427917No Hit
AAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCC135600.10801012140863359No Hit
GGGAGGCCTCCACCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGC134420.10707021032262926No Hit
CAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCG133560.10638519037859219No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA132040.10517445745424764No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT59200.039.9668461
CTGACTC77650.030.1928752
ACTCTAG86300.028.421295
GACTCTA90250.027.7976764
CTAGCAG90700.026.4256088
TGACTCT96150.025.0007273
CTCTAGC101950.023.9900786
TAGCAGA104700.023.0603879
CTATCGA108550.021.46858417
TCTATCG112100.020.757516
GATCTAT124250.018.9255814
GCAGATC125550.018.89654411
GTGCGCA173050.018.76854168
AGCAGAT129000.018.68947810
TGCGCAT177500.018.31765269
ATCTATC128350.018.18477615
CCCTACA16400.018.13605110
AGATCTA130700.018.12547513
GGTGCGC179950.018.06854667
TATCGAT132550.017.68734718
Produced by FastQC (version 0.11.2)