FastQCFastQC Report
Thu 30 Jun 2016
H7L3YBGXY_n01_evbztga_19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7L3YBGXY_n01_evbztga_19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28792473
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG955240.33176726431244724No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC944630.3280822734469526No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT893080.31017828861036006No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA690120.23968764336429177No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG509090.17681357207489612No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC481210.16713048580439757No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG477530.16585237398677077No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG439990.15281424419500195No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC401290.1393732313302855No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC383640.13324315698759187No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG370110.12854401217984993No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA367540.1276514177854747No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA353560.12279598213046862No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC344820.119760466563605No Hit
CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGGATG341740.11869074254233043No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT327200.11364081161072896No Hit
GTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCAT324500.11270306652714408No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT315160.1094591631639283No Hit
GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAA313440.10886178481438534No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG308520.10715300488429737No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT306580.10647921767609195No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG288170.10008518545801884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT152650.041.5012051
CTGACTC194050.032.7107852
ACTCTAG215350.030.1074315
GACTCTA227650.029.2185334
TGACTCT233550.027.6565973
CTAGCAG232250.027.5253458
CTCTAGC256350.025.510696
TAGCAGA271750.023.6154969
CTATCGA287350.022.06560317
TCTATCG294800.021.60315916
GCAGATC319850.019.86755211
GATCTAT322200.019.75560414
AGCAGAT327200.019.63508210
ATCTATC335750.019.02095215
AGATCTA336200.018.91212713
TATCGAT337650.018.84094818
CCCTACA36450.018.33619310
TTCTAGC367750.017.25650224
TAGCATG375650.016.86622427
ATTCTAG379050.016.80605923
Produced by FastQC (version 0.11.2)