FastQCFastQC Report
Thu 30 Jun 2016
H7L3YBGXY_n01_evbztga_18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7L3YBGXY_n01_evbztga_18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25313404
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC619210.24461743667505167No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG492030.19437528038504817No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT446590.17642431653996435No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA383560.15152446506206751No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG370980.14655476600460374No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG274410.10840501735760232No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC260850.10304817163270495No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG254450.10051986686579173No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT111000.031.4381961
CTAGCAG132550.026.292518
CTGACTC144050.024.5351582
ACTCTAG152850.023.7389955
TATGCCG37100.023.30475248
GACTCTA162500.022.8677584
GTATGCC38600.022.8520147
CGTATGC38050.022.8140446
TAGCAGA173850.020.429539
CTATCGA172000.019.91715417
GCACACG48450.019.7169711
TGACTCT181600.019.6920873
CTCTAGC186950.019.6522376
TCTATCG184550.018.71433616
ATGCCGT50400.017.50146349
GATCTAT205900.016.87622814
CTCGTAT51650.016.60289244
GTCACGT54200.016.27651229
TCGTATG54700.016.25306345
TGCCGTC55350.016.12642350
Produced by FastQC (version 0.11.2)