FastQCFastQC Report
Thu 30 Jun 2016
H7L3YBGXY_n01_evbztga_17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7L3YBGXY_n01_evbztga_17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17996442
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC569080.3162180613256776No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG497120.2762323797115007No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG361460.20085081262173932No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT349970.19446621726672417No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA301810.1677053719840844No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG258410.14358949396775209No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG243650.13538787278063075No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC240360.13355973364068296No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG232490.12918664700500243No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC213060.11839006843686103No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC207650.11538391866570069No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA195180.10845477122644577No Hit
CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGGATG192360.10688779482077625No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT190510.10585981384542567No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT186550.10365937889278336No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT182010.10113665801273385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT75500.034.7227361
CTAGCAG101450.025.936838
CTGACTC102000.025.4216752
ACTCTAG110450.025.0270045
GACTCTA116000.024.0106454
TGACTCT126000.021.2722873
TAGCAGA125250.021.1764649
CTCTAGC136350.020.2475746
CTATCGA132200.019.58723417
TCTATCG138000.019.04271516
CCCTACA24100.019.02040910
GTGCGCA205700.017.74711868
TGCGCAT214700.017.10070669
GGTGCGC215600.016.89968367
GATCTAT155200.016.81981314
ATCTATC155900.016.6995415
TCCCTAC28750.016.430779
GCAGATC161850.016.21489511
AGGTGCG226050.016.19621366
AGCAGAT165500.016.06871610
Produced by FastQC (version 0.11.2)