FastQCFastQC Report
Thu 30 Jun 2016
H7L3YBGXY_n01_evbztga_16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7L3YBGXY_n01_evbztga_16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30172919
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC932220.30895916964480635No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG792890.26278199997819235No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG605670.20073298178409585No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT590530.19571523722978212No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA473040.1567763463654279No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG403300.1336629048054648No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC394940.13089220834086354No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG388260.1286783025533592No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG351390.11645873572921467No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC347070.11502698827382263No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC335530.11120236659900225No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA317780.1053196079570558No Hit
CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGGATG310290.10283724952166544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT135350.033.1290441
CTAGCAG170550.026.42518
CTGACTC173900.025.6367322
ACTCTAG194750.024.2191665
GACTCTA205500.023.5142694
TAGCAGA213600.021.361969
TGACTCT218850.021.0089443
CTATCGA223450.019.90395217
CTCTAGC240400.019.7804456
TCTATCG234200.018.97543116
GATCTAT260600.017.22791914
ATCTATC265650.016.99279415
GTGCGCA336750.016.51512368
GCAGATC273900.016.42934211
AGCAGAT280400.016.3354610
AGATCTA277500.016.24169313
TATCGAT275750.016.07830218
TGCGCAT350450.015.97912469
GGTGCGC353250.015.74374267
GCATGAC290000.015.28200729
Produced by FastQC (version 0.11.2)