FastQCFastQC Report
Thu 30 Jun 2016
H7L3YBGXY_n01_evbztga_15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7L3YBGXY_n01_evbztga_15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49952033
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGG592820.1186778524109319No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACA70750.016.32137910
TCCCTAC84850.014.392629
CCTACAC108550.010.89573911
CACGAGT382700.010.88293768
GGATAAC421350.010.8740251
GGCCACG390800.010.49595565
GTACAGC208250.010.27211633
ATGCCGC209600.010.1364746
GCCAAAT374000.09.9484481
GGTACCT218450.09.90075721
AACGGCC423950.09.89850362
TCGGCGC216800.09.81545870
GCCACGA426850.09.79840766
CGGGCAT42550.09.7890621
GAACGGC429600.09.581161
CATGCGC425700.09.55505428
CGCGGGG28250.09.5403861
GGCCATC436250.09.53708710
CCGGGGT33050.09.5315761
GGGCTCC432550.09.50863652
Produced by FastQC (version 0.11.2)