FastQCFastQC Report
Thu 30 Jun 2016
H7L3YBGXY_n01_evbztga_14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7L3YBGXY_n01_evbztga_14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38942886
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGG519210.13332602005922212No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACA52900.015.87538510
GGATAAC339850.012.5750341
TCCCTAC67750.012.5504719
CACGAGT338700.011.37708968
GGCCACG346450.011.0012665
CGGGGGG29850.010.7875861
ACATGGC377400.010.7826876
GGCCATC361100.010.74664110
GCGGGGG36550.010.7253491
CATGGCC366300.010.7082487
CGGGCAT35100.010.570111
CGCGGGG23500.010.42583751
CATGCGC365800.010.40195928
GCCACGA372700.010.35830166
GCGGGCT31550.010.3172591
AACGGCC377050.010.30361862
ATGGCCA380450.010.2916238
ATGCGCT377350.010.26968529
GAACGGC378200.010.20782361
GATAACA414850.010.1897092
Produced by FastQC (version 0.11.2)