FastQCFastQC Report
Thu 30 Jun 2016
H7L3YBGXY_n01_evbztga_13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7L3YBGXY_n01_evbztga_13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22737013
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGG274920.12091298008230017No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACA32100.014.9339510
CGCGGGG14100.013.1563211
TCCCTAC38400.012.7571929
GGATAAC183950.011.4163981
GGGCGCT10300.010.8740261
CCGGGAT57700.010.6154861
GCCGGGG23600.010.5298941
GGGGTAT17950.010.5294811
GCCGGGT27850.010.3054341
CTACACG43650.010.26097812
CCTACAC48050.010.19522311
CGGGCAT19450.010.0773441
GGCCACG187150.010.061028565
CCGGGGT18600.09.9733381
CACGAGT191750.09.9660668
CATGGCC201650.09.8904667
GGGGCAT20550.09.8785641
GGGGAAT72450.09.8552911
CGGGGGT17050.09.8535911
CGGGGGG17050.09.8535911
Produced by FastQC (version 0.11.2)