FastQCFastQC Report
Wed 9 Mar 2022
H7L2KDMXY_n02_CIVR_Y3_P3_F2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7L2KDMXY_n02_CIVR_Y3_P3_F2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2257
Sequences flagged as poor quality0
Sequence length151
%GC64

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCACCGACTGCCCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTG1245.494018608772707Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG411.8165706690296854No Hit
ATATCTCTGTTGCACGCCGGAGTTCATAAGAGATATTAAGGCCCTCGATG170.7532122286220646No Hit
GCCTTAATATCTCTGTTGCACGCCGGAGTTCATAAGAGATATTAAGGCCC150.664599025254763No Hit
ATCACCGACTGCCCATAGAGAGGAAAGCGGAGGCGTAGTGGAGATCGGAA140.6202924235711121ABI Solid3 Adapter B (100% over 41bp)
CTGCCCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACTTGTAG120.5316792202038104Illumina Single End PCR Primer 1 (96% over 33bp)
CCTTAATATCTCTGTTGCACGCCGGAGTTCATAAGAGATATTAAGGCCCT100.4430660168365086No Hit
CCACTACGCCTCCGCTTTCCTCTCTATGGGCAGTCGGTGATAGATCGGAA100.4430660168365086ABI Solid3 Adapter B (100% over 41bp)
ATCACCGACTGCCCATAGAGAGGAGATCGGAAGAGCGTCGTGTAGGGAAA80.35445281346920693Illumina Single End PCR Primer 1 (100% over 27bp)
CCCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACTTGTAGTGT80.35445281346920693Illumina Single End PCR Primer 1 (96% over 33bp)
AGCTTCAATGCCTTTTTCCGCAGATTGTTGACGACGCTTACCTTCAAAGT70.31014621178555607No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACTTGTAGTGTAGATC70.31014621178555607Illumina Single End PCR Primer 1 (96% over 33bp)
ACTGCCCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACTTGTA70.31014621178555607Illumina Single End PCR Primer 1 (96% over 33bp)
GGAACAGCACCTCTCATCAAGTTCCATCTTCCCCCACATGCCGTCGACTC40.17722640673460346No Hit
ATCACCGGACTGCCCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT40.17722640673460346Illumina Single End PCR Primer 1 (100% over 33bp)
CTTGGGCCATCTCAGTAAAGTTCCCTGTACTTCCAGAGGGCAAAGAGCTC30.1329198050509526No Hit
AATTAATATTTTCAATTGCGTCATGAATAATTGAATACAGGTATCAGGTA30.1329198050509526No Hit
ACACTCTTTCCCATCACGAACTGCGATCTGTCTTCCAAGGAGTAGCTGTG30.1329198050509526No Hit
CCAAGTTTGACTAACTTCTCGGCCAAAAGATGCCGTTGCCGGCTTCTTAA30.1329198050509526No Hit
GCTGACATGAGTAGCGACAAACAGTGCGAGAAACACTGTCGCCGAAAGTC30.1329198050509526No Hit
ACACTCTTACCCTACGCCTCCGCTTTCCTCTCTATGGGCAGTCGGTGATA30.1329198050509526ABI Solid3 Adapter B (100% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.1329198050509526No Hit
ATCATGCCCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACTTG30.1329198050509526Illumina Single End PCR Primer 1 (96% over 33bp)
GGAAAATTGAAACGAAGATAATATTTTACCAAAGGGTTAGTAATAATTGT30.1329198050509526No Hit
ATCACCGACTGCCCATAGATCGGAAGAGCGTCGTGGAGGGAAAGAGTGTG30.1329198050509526Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)