FastQCFastQC Report
Wed 9 Mar 2022
H7L2KDMXY_n02_CIVR_Y3_P3_E1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7L2KDMXY_n02_CIVR_Y3_P3_E1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3154
Sequences flagged as poor quality0
Sequence length151
%GC68

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1715.421686746987952No Hit
ATCACCGACTGCCCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT1404.438807863031072Illumina Single End PCR Primer 1 (96% over 33bp)
GCCTTAATATCTCTGTTGCACGCCGGAGTTCATAAGAGATATTAAGGCCC491.5535827520608751No Hit
ATATCTCTGTTGCACGCCGGAGTTCATAAGAGATATTAAGGCCCTCGATG381.2048192771084338No Hit
CCTTAATATCTCTGTTGCACGCCGGAGTTCATAAGAGATATTAAGGCCCT371.173113506658212No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT310.9828788839568802No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGG300.9511731135066582No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG240.7609384908053266No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGT170.538998097653773No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTG150.4755865567533291No Hit
ATCACCGACTGCCCATAGAGAGGAAAGCGGAGGCGTAGTGGAGATCGGAA150.4755865567533291ABI Solid3 Adapter B (100% over 41bp)
TATCTCTGTTGCACGCCGGAGTTCATAAGAGATATTAAGGCCCTCGATGG130.4121750158528852No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGG100.31705770450221943No Hit
CCACTACGCCTCCGCTTTCCTCTCTATGGGCAGTCGGTGATAGATCGGAA100.31705770450221943ABI Solid3 Adapter B (100% over 41bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGG100.31705770450221943No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGT90.2853519340519975No Hit
CTGCCCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCGTCCGG80.2536461636017755Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTG80.2536461636017755No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGTGG70.22194039315155356No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGTG70.22194039315155356No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAG70.22194039315155356No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGG70.22194039315155356No Hit
AATATCTCTGTTGCACGCCGGAGTTCATAAGAGATATTAAGGCCCTCGAT70.22194039315155356No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGG60.19023462270133165No Hit
ATCTCTGTTGCACGCCGGAGTTCATAAGAGATATTAAGGCCCTCGATGGA60.19023462270133165No Hit
CCCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCGTCCGGTGT60.19023462270133165Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGG60.19023462270133165No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGA50.15852885225110971No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGTGG50.15852885225110971No Hit
CAGAGGAGCAGGACGGAGACGGGCAGAGGAGGAGTGAAATGCGATTCACA50.15852885225110971No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGG50.15852885225110971No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGT50.15852885225110971No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGTGG50.15852885225110971No Hit
CTTCCGATCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCGTCCG50.15852885225110971Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCGTCCGGTGTAGATC50.15852885225110971Illumina Single End PCR Primer 1 (96% over 33bp)
CTTAATATCTCTGTTGCACGCCGGAGTTCATAAGAGATATTAAGGCCCTC50.15852885225110971No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.15852885225110971No Hit
TAATATCTCTGTTGCACGCCGGAGTTCATAAGAGATATTAAGGCCCTCGA40.12682308180088775No Hit
ATCACCGACTGCCCATAGAGAGGAGATCGGAAGAGCGTCGTGTAGGGAAA40.12682308180088775Illumina Single End PCR Primer 1 (100% over 27bp)
ATCACCGACTGCCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTC40.12682308180088775Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)