FastQCFastQC Report
Wed 9 Mar 2022
H7L2KDMXY_n02_CIVR_Y3_P2_D6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7L2KDMXY_n02_CIVR_Y3_P2_D6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27005
Sequences flagged as poor quality0
Sequence length151
%GC73

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACACACGGACTTACGGCCCACCCACAGATTCGCATCCACTCAGCTC755327.96889464913905No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGTGCTAGTGTAGATC5371.988520644325125Illumina Single End PCR Primer 1 (97% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4211.5589705610072209No Hit
CGCACATTCCGCTTCTACCGGGGGGGTCGAGCTGAGTGGATGCGAATCTG2680.9924088131827439No Hit
TCGCACATTCCGCTTCTACCGGGGGGGTCGAGCTGAGTGGATGCGAATCT2530.9368635437881874No Hit
TCGCACACACGGACTTACGGCCCACCACAGATTCGCATCCACTCAGCTCG630.23329013145713756No Hit
GCACATTCCGCTTCTACCGGGGGGGTCGAGCTGAGTGGATGCGAATCTGT550.20366598778004072No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG500.18515089798185522No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGT450.1666358081836697No Hit
TCGCACACACGGACTTACGGCCCACCCACAGATCGCATCCACTCAGCTCG420.15552675430475837No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA390.14441770042584706No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTG380.14071468246620997No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT380.14071468246620997No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTGT370.13701166450657284No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGT320.11849657470838733No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGG310.11479355674875025No Hit
TCGCACACACGGACTTACGGCCCACCCCAGATTCGCATCCACTCAGCTCG280.10368450286983892No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGG280.10368450286983892No Hit
TCGCACACACGGACTTACGGCCACCCACAGATTCGCATCCACTCAGCTCG280.10368450286983892No Hit
TCGCACACACGGACTTACGGCCCACCCACGATTCGCATCCACTCAGCTCG280.10368450286983892No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)