FastQCFastQC Report
Wed 9 Mar 2022
H7L2KDMXY_n02_CIVR_Y3_P1_D12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7L2KDMXY_n02_CIVR_Y3_P1_D12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17004363
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA1089250.640570893481867No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC1034730.6085085339568439No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC910340.5353567199194701No Hit
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGT620040.3646358290516381No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC565150.3323558783119368No Hit
GTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCA510090.2999759532303562No Hit
CGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGT314040.1846820136690801No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT300740.1768604916279428No Hit
CGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCC282800.16631025813786732No Hit
AAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAG275970.1622936419317795No Hit
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACG250720.14744451174089848No Hit
CGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTAC245620.14444528148452254No Hit
GCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGG237110.13944068354692263No Hit
GTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGC231160.1359415815811507No Hit
GGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGAC230080.13530645046803574No Hit
CAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAA228780.13454194079484189No Hit
CTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCC223930.13168973162946473No Hit
CAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTC220700.12979021913375996No Hit
CGCACGTGGACGACATGCCCAACGCGCTGTCCGCCCTGAGCGACCTGCAC218480.12848467184569043No Hit
CAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTGACTC216660.12741435830321898No Hit
CGCGCACAAGCTTCGGGTGGACCCGGTCAACTTCAAGCTCCTAAGCCACT211490.12437396214136337No Hit
GGAACGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGT208610.12268027917305695No Hit
GTCCAGGTGAGCCAGGCCATCACTAAAGGCACCGAGCACTTTCTTGCCAT208180.12242740289653896No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTC198780.11689940987498326No Hit
GAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGG194290.11425891108064441No Hit
CCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGG187340.11017172475087716No Hit
CTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCA182860.10753710680017829No Hit
CTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTGAT181460.10671378869058488No Hit
GGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTAAAGG180830.10634329554126784No Hit
GGACGACATGCCCAACGCGCTGTCCGCCCTGAGCGACCTGCACGCGCACA173240.10187973521854361No Hit
GCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGTCG173020.10175035665846464No Hit
CCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGT172610.1015092420692266No Hit
GTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGA170590.1003213116539561No Hit
GTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTGACTCCTGAGGA170360.10018605225023719No Hit
CGTGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTGGTCTACC170130.10005079284651827No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)