FastQCFastQC Report
Wed 9 Mar 2022
H7L2KDMXY_n01_CIVR_Y3_P3_F2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7L2KDMXY_n01_CIVR_Y3_P3_F2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2257
Sequences flagged as poor quality0
Sequence length151
%GC62

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGAAATCTCGTAT36516.171909614532566TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGAAATCGCGTAT1888.329641116526362TruSeq Adapter, Index 7 (97% over 35bp)
ATGGGCAGTCGGTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1275.62693841382366Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGAAATCTCGGAT582.5697828976517503TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGAAATCTCGTTT562.4811696942844486TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGAAATCGCGTTT431.9051838723969872TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGAAATCGCGGAT291.2848914488258751TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGAAATCTCGGTT210.930438635356668TruSeq Adapter, Index 7 (97% over 35bp)
ATATCTCTTATGAACTCCGGCGTGCAACAGAGGATCCATCGAGGGCCTTA170.7532122286220646No Hit
GCCTTAATATCTCTTATGAACTCCGGCGTGCAACAGAGGATCCATCGAGG150.664599025254763No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGAAATCGCGGTT130.5759858218874613TruSeq Adapter, Index 7 (97% over 35bp)
CCACTACGCCTCCGCTTTCCTCTCTATGGGCAGTCGGTGATAGATCGGAA130.5759858218874613ABI Solid3 Adapter B (100% over 41bp)
ATCACCGACTGCCCATAGAGAGGAAAGCGGAGGCGTAGTGGAGATCGGAA100.4430660168365086ABI Solid3 Adapter B (100% over 41bp)
CCTTAATATCTCTTATGAACTCCGGCGTGCAACAGAGGATCCATCGAGGG100.4430660168365086No Hit
ATGGGCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGAA100.4430660168365086TruSeq Adapter, Index 7 (97% over 35bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGAAATCTCGTA80.35445281346920693TruSeq Adapter, Index 9 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGAAATCGCGTGT80.35445281346920693TruSeq Adapter, Index 7 (97% over 35bp)
ATGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGAAATC80.35445281346920693TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGAAATCTCGTGT70.31014621178555607TruSeq Adapter, Index 7 (97% over 35bp)
CCTCTCTATGGGCAGTCGGTGATAGATCGGAAGAGCACACGTCTGAACTC70.31014621178555607Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATGGGCAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGA60.2658396101019052TruSeq Adapter, Index 7 (97% over 35bp)
CGTCACTGCGGGTATGGGTGGCGGTACAGGTACAGGTGCTGCCCCTGTGG50.2215330084182543No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGAAATCGCGGGT50.2215330084182543TruSeq Adapter, Index 7 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGAAATCTCGTA50.2215330084182543TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGAAATCTCGTAA50.2215330084182543TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGAAATCTCGGGT50.2215330084182543TruSeq Adapter, Index 7 (97% over 35bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGAAATCGCGTA40.17722640673460346TruSeq Adapter, Index 9 (97% over 35bp)
AAACGAGTAAAAATCAATGGTACAAGACATTAAAAGAAGGGTTTGAATCG40.17722640673460346No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGAAATCTCGTATG40.17722640673460346TruSeq Adapter, Index 7 (97% over 34bp)
ATGGGCAGTCCGGTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA40.17722640673460346Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATGGGCAGTCGGTGATGATCGGAAGAGCACACGTCTGAACTCCAGTCACG40.17722640673460346Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
CACCAGCTCGCGTTGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCG30.1329198050509526No Hit
AACCGGAAAATTTTGTTTTTGCAGATGAATATCACAAAGAATTATCAATA30.1329198050509526No Hit
ATGGGCATGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGT30.1329198050509526TruSeq Adapter, Index 7 (97% over 35bp)
GCAATGCTGACGGGACGTGCGTTTTCCTCAGCAAACTGGCAAGCTCCCTG30.1329198050509526No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG30.1329198050509526No Hit
GATCGGAAGAGCACACGTCTGAACTCAAGTCACGAAGTGAAATCTCGTAT30.1329198050509526TruSeq Adapter, Index 9 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGAAATCTCGGAA30.1329198050509526TruSeq Adapter, Index 7 (97% over 35bp)
GCCACAGATAAAAGTAAACCAACACTAAATAAAAATAGTTCTAGAGTCTA30.1329198050509526No Hit
ATCACCGACTGCCCATAGAGAGGAAAGCGGAGGCGTAGGGTAAGAGTGTA30.1329198050509526ABI Solid3 Adapter B (97% over 40bp)
GTCGAAAAGCCTGTTCAATCCGATAAGGACAGACAACATTTCTCATTCGT30.1329198050509526No Hit
ATGGGCAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTG30.1329198050509526TruSeq Adapter, Index 7 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)