FastQCFastQC Report
Wed 9 Mar 2022
H7L2KDMXY_n01_CIVR_Y3_P3_E1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7L2KDMXY_n01_CIVR_Y3_P3_E1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3154
Sequences flagged as poor quality0
Sequence length151
%GC63

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGAGGGATCTCGTAT69221.940393151553582TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGAGGGATCGCGTAT3149.95561192136969TruSeq Adapter, Index 7 (97% over 35bp)
ATGGGCAGTCGGTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1444.56563094483196Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGAGGGATCTCGGAT541.7121116043119846TruSeq Adapter, Index 7 (97% over 35bp)
GCCTTAATATCTCTTATGAACTCCGGCGTGCAACAGAGGATCCATCGAGG481.5218769816106532No Hit
ATATCTCTTATGAACTCCGGCGTGCAACAGAGGATCCATCGAGGGCCTTA371.173113506658212No Hit
CCTTAATATCTCTTATGAACTCCGGCGTGCAACAGAGGATCCATCGAGGG341.077996195307546No Hit
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGAGGGATCTCGTA290.9194673430564362TruSeq Adapter, Index 9 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGAGGGATCGCGGAT230.7292327203551047TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGAGGGATCTCGTTT200.6341154090044389TruSeq Adapter, Index 7 (97% over 35bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGAGGGATCGCGTA160.507292327203551TruSeq Adapter, Index 9 (97% over 35bp)
CCACTACGCCTCCGCTTTCCTCTCTATGGGCAGTCGGTGATAGATCGGAA150.4755865567533291ABI Solid3 Adapter B (100% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGAGGGATCGCGTTT120.3804692454026633TruSeq Adapter, Index 7 (97% over 35bp)
TATCTCTTATGAACTCCGGCGTGCAACAGAGGATCCATCGAGGGCCTTAA110.3487634749524413No Hit
ATCACCGACTGCCCATAGAGAGGAAAGCGGAGGCGTAGTGGAGATCGGAA100.31705770450221943ABI Solid3 Adapter B (100% over 41bp)
ATGGGCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGAGGG100.31705770450221943TruSeq Adapter, Index 7 (97% over 35bp)
AATATCTCTTATGAACTCCGGCGTGCAACAGAGGATCCATCGAGGGCCTT100.31705770450221943No Hit
CTTAATATCTCTTATGAACTCCGGCGTGCAACAGAGGATCCATCGAGGGC80.2536461636017755No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGAGGGATCTCGTA60.19023462270133165TruSeq Adapter, Index 7 (97% over 35bp)
ATCTCTTATGAACTCCGGCGTGCAACAGAGGATCCATCGAGGGCCTTAAT60.19023462270133165No Hit
GATCGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGAGG50.15852885225110971TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGAGGGATCTCGTATG50.15852885225110971TruSeq Adapter, Index 7 (97% over 34bp)
ATGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGAGGGATC50.15852885225110971TruSeq Adapter, Index 7 (97% over 35bp)
GTCTCTCACTTCACACAGGCTCAATAATGTCGACTGACGCGGCTGTGAAT50.15852885225110971No Hit
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGAGGGATCTCGGA40.12682308180088775TruSeq Adapter, Index 9 (97% over 35bp)
CCTGCAGATGCATCCAGTACATGATCTCTTGTTGGTAAGTTGCAATCGGA40.12682308180088775No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGAGGGATCGCGTATG40.12682308180088775TruSeq Adapter, Index 7 (97% over 34bp)
ATGGCAGTCGGTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG40.12682308180088775Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGAGGGATCTCGTAA40.12682308180088775TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTTCCCTACACGACGCTCTTC40.12682308180088775Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
TTAATATCTCTTATGAACTCCGGCGTGCAACAGAGGATCCATCGAGGGCC40.12682308180088775No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)