FastQCFastQC Report
Wed 9 Mar 2022
H7L2KDMXY_n01_CIVR_Y3_P2_H12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7L2KDMXY_n01_CIVR_Y3_P2_H12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6864
Sequences flagged as poor quality0
Sequence length151
%GC70

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCTCGTAT274740.02039627039627TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCGCGTAT157923.004079254079254TruSeq Adapter, Index 10 (97% over 38bp)
TCGCACATTCCGCTTCTACCGGGGGGGTCGAGCTGAGTGGATGCGAATCT1452.1124708624708624No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCTCGTA1371.995920745920746TruSeq Adapter, Index 10 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCGCGTA881.282051282051282TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCTCGGAT751.0926573426573427TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCGCGGAT480.6993006993006993TruSeq Adapter, Index 10 (97% over 38bp)
TCGCACACACGGACTTACGGCCCACCCACAGATTCGCATCCACTCAGCTC460.6701631701631702No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCTCGTTT420.6118881118881119TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCGCGTTT340.49533799533799533TruSeq Adapter, Index 10 (97% over 38bp)
CGCACATTCCGCTTCTACCGGGGGGGTCGAGCTGAGTGGATGCGAATCTG260.3787878787878788No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCTCGTATG170.24766899766899766TruSeq Adapter, Index 10 (97% over 37bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCGCGTA130.1893939393939394TruSeq Adapter, Index 10 (97% over 35bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCTCGTA130.1893939393939394TruSeq Adapter, Index 10 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCGCGTAA100.1456876456876457TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCTCGTAA90.13111888111888112TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCTGGTAT90.13111888111888112TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCGGTGATCTCGTAT90.13111888111888112TruSeq Adapter, Index 10 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCGCGTGT90.13111888111888112TruSeq Adapter, Index 10 (97% over 38bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCTCGTAT80.11655011655011654TruSeq Adapter, Index 10 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCTCGTGT70.10198135198135197TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCGGGTAT70.10198135198135197TruSeq Adapter, Index 10 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)