FastQCFastQC Report
Wed 9 Mar 2022
H7L2KDMXY_n01_CIVR_Y3_P2_D6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7L2KDMXY_n01_CIVR_Y3_P2_D6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27005
Sequences flagged as poor quality0
Sequence length151
%GC68

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGGAACATCTCGTAT837731.020181447880024TruSeq Adapter, Index 25 (97% over 37bp)
TCGCACATTCCGCTTCTACCGGGGGGGTCGAGCTGAGTGGATGCGAATCT694325.710053693760415No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGGAACATCGCGTAT537019.88520644325125TruSeq Adapter, Index 25 (97% over 37bp)
TCGCACACACGGACTTACGGCCCACCCACAGATTCGCATCCACTCAGCTC6152.277356045176819No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGGAACATCTCGTA3601.3330864654693575TruSeq Adapter, Index 25 (97% over 37bp)
CGCACATTCCGCTTCTACCGGGGGGGTCGAGCTGAGTGGATGCGAATCTG3531.3071653397518979No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGGAACATCGCGTA1750.6480281429364932TruSeq Adapter, Index 25 (97% over 37bp)
TCGCGCACATTCCGCTTCTACCGGGGGGGTCGAGCTGAGTGGATGCGAAT1050.38881688576189594No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGGAACATCTCGTATG720.2666172930938715TruSeq Adapter, Index 25 (97% over 36bp)
GCACATTCCGCTTCTACCGGGGGGGTCGAGCTGAGTGGATGCGAATCTGT640.23699314941677466No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGGAACATCGCGTATG590.21847805961858913TruSeq Adapter, Index 25 (97% over 36bp)
TCGCACATTCCGCTTCTACCGGGGGGGTCGAGCTGAGTGGATGCGAATCG540.19996296982040365No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA490.1814478800222181No Hit
TCGCACATTCCGCTTCTACCGGGGGGTCGAGCTGAGTGGATGCGAATCTG450.1666358081836697No Hit
TCGCACATTCCGCTTCTACCGGGGGGGTCGAGCTGAGTGGATGCGATCTG410.15182373634512128No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGGAACAGCTCGTAT350.12960562858729863TruSeq Adapter, Index 25 (97% over 37bp)
TCGCACATTCCGCTTCTACCGGGGGGGTCGAGCTGAGTGGATGCGACTCT310.11479355674875025No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGGAACAGCGCGTAT300.11109053878911312TruSeq Adapter, Index 25 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)