FastQCFastQC Report
Wed 9 Mar 2022
H7L2KDMXY_n01_CIVR_Y3_P2_D12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7L2KDMXY_n01_CIVR_Y3_P2_D12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27
Sequences flagged as poor quality0
Sequence length151
%GC60

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCACCTATCTCGTAT414.814814814814813TruSeq Adapter, Index 10 (97% over 37bp)
GCCGCAGAGGCTTGGGCTGCAGAAAAAGGATCCTGTTTGCTGTGTAATCC311.11111111111111No Hit
CTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGACT27.4074074074074066No Hit
ATCCACGTTCACCTTGCCCCACAGGGCAGTAACGGCAGACTTCTCCAGAT27.4074074074074066No Hit
GGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCAGCTTAACG27.4074074074074066No Hit
GAAGTTGGTGGTGGATCTGTCCACTCCTGATGCTGTTATGGGCAACCCTA13.7037037037037033No Hit
GCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCG13.7037037037037033No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCACCTATCGCGTAT13.7037037037037033TruSeq Adapter, Index 10 (97% over 37bp)
GCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTC13.7037037037037033No Hit
GTTAACAGTGCCTTTTCGATGGAATCACACTTTTTGGCTGTCACCTTGTG13.7037037037037033No Hit
GTGGTATTTTAAAAAATCTCTCCCAAATTTGATGACATAGGGACAGTGGT13.7037037037037033No Hit
GACGGGATCATCCTGTAGACGAGGGAGTCATCAACGGAACTGTTGTACTG13.7037037037037033No Hit
CTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCC13.7037037037037033No Hit
GGTTGTACCCCTCGAGGCCCATCCAGGAAAGGCAGGTGAGCAGGGAGAAG13.7037037037037033No Hit
TCAGGGGTCAGCAGCTTGTGCTCAATGAAATACTGGATACCCTTGGCGGG13.7037037037037033No Hit
GCGACCTGCACGCGCACAAGCTTCGGGTGGACCCGGTCAACTTCAAGCTC13.7037037037037033No Hit
CGAGGTGAGACTAAGAATCTGGAGAAAGAGCATCAGTCTGATGTAGCTTT13.7037037037037033No Hit
GGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCAGCTTAACGG13.7037037037037033No Hit
ATTTTGCACTTCAACCAGATGACGTGTACTACTGTGGAATAAAGTACATC13.7037037037037033No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)